Comparison of the Efficiency of Systemic and Local Cell Therapy with Human Umbilical Cord Blood Mononuclear Cells in Rats with Severe Spinal Cord Injury

Bull Exp Biol Med. 2020 Feb;168(4):552-555. doi: 10.1007/s10517-020-04751-7. Epub 2020 Mar 10.

Abstract

Rat model of severe contusion spinal cord injury was used to study the effect of single intravenous and intraspinal injection of human umbilical cord blood mononuclear cells on the restoration of motor function of the hind limbs. Recovery of the motor function of the hind limbs was assessed using load tests and open-field test according to BBB scale. Cell injection via both routes significantly improved (p≤0.05) the recovery of the motor function of the hind limbs by 35-40% relative to the level of "self-recovery"; the effects of intravenous andintraspinal administration did not differ significantly.

Keywords: cell therapy; human umbilical cord blood mononuclear cells; spinal cord injury.

Publication types

  • Comparative Study

MeSH terms

  • Acute Disease
  • Animals
  • Cell- and Tissue-Based Therapy / methods*
  • Cryopreservation / methods
  • Female
  • Fetal Blood / cytology*
  • Fetal Blood / physiology
  • Hindlimb
  • Humans
  • Injections, Intralesional
  • Injections, Intravenous
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / physiology
  • Leukocytes, Mononuclear / transplantation*
  • Motor Activity / physiology*
  • Primary Cell Culture
  • Rats
  • Rats, Sprague-Dawley
  • Recovery of Function*
  • Spinal Cord / surgery
  • Spinal Cord Injuries / pathology
  • Spinal Cord Injuries / surgery
  • Spinal Cord Injuries / therapy*
  • Transplantation, Heterologous