Data on the optimization and validation of HPLC-PDA method for quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof

Anna Marchelak; Monika Anna Olszewska; Aleksandra Owczarek

doi:10.1016/j.dib.2020.105319

Data on the optimization and validation of HPLC-PDA method for quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof

Data Brief. 2020 Feb 22:29:105319. doi: 10.1016/j.dib.2020.105319. eCollection 2020 Apr.

Authors

Anna Marchelak¹, Monika Anna Olszewska¹, Aleksandra Owczarek¹

Affiliation

¹ Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszynskiego St., 90-151, Lodz, Poland.

Abstract

This paper presents data on the optimization and validation of an RP-HPLC-PDA method for quantification of 30 phenolic constituents of the blackthorn (Prunus spinosa L.) flower. The method development data cover detailed descriptions of the optimization process in terms of elution solvents, gradient profile, temperature, and flow rate. The validation data cover accuracy and precision (intra- and inter-day variability) for retention times and peak areas. Moreover, the quantification data for the commercial samples of blackthorn flower (different manufactures and years of collection), as well as for the extracts (of different polarity) prepared thereof, are included. The data presented here were related to the article: "Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control" [1].

Keywords: High performance liquid chromatography; Optimization; Polyphenols; Prunus spinosa; Quality control; Quantification; Tertiary gradient; Validation.