Nutrition assessment of vitamin A and vitamin D in northeast Chinese population based-on SPE/UPLC/PDA

Maoqing Wang; Hongyu Chen; Shanshan Du; Xinxin Guo; Jiali Zhao; Changhao Sun; Ying Li

doi:10.1186/s40795-018-0219-x

Nutrition assessment of vitamin A and vitamin D in northeast Chinese population based-on SPE/UPLC/PDA

BMC Nutr. 2018 Mar 27:4:12. doi: 10.1186/s40795-018-0219-x. eCollection 2018.

Authors

Maoqing Wang¹, Hongyu Chen¹, Shanshan Du¹, Xinxin Guo¹, Jiali Zhao¹, Changhao Sun¹, Ying Li¹

Affiliation

¹ National Key Disciplines of Nutrition and Food Hygiene, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin 150081 People's Republic of China.

Abstract

Background: The aims of the current study were to assess the nutritional status of 25OHD3 and retinol in a northern Chinese population using our established reliable method for the simultaneous determination of serum 25OHD3 and retinol.

Method: We established a reliable method for the simultaneous determination of 25OHD3 and retinol using SPE and UPLC/PDA; measured the serum levels of 25OHD3 and retinol in elementary school students, middle school students, and adults (n = 1181) in northern China; and assessed their nutritional status.

Results: Our method had good precision, detection limit, and linear quantitative range and could process 100 samples within 12 h. The average levels of 25OHD3 and retinol were 16.1 ± 6.7 ng/ml and 328.1 ± 117.1 ng/ml, respectively, in all samples. VD deficiency was common, with a prevalence > 60% in all three age groups, and the high prevalence of VA deficiency (26.1%) was observed only in the elementary school students.

Conclusions: Vitamin A supplementation should be considered for elementary school students, and vitamin D supplementation is highly recommended for all age groups in Harbin. Our method could be widely adopted in population-based studies and clinical practice.