Characterization and Neuroprotection Potential of Seleno-Polymannuronate

Decheng Bi; Xiaofan Li; Tong Li; Xiuting Li; Zhijian Lin; Lijun Yao; Hui Li; Hong Xu; Zhangli Hu; Zhenqing Zhang; Qiong Liu; Xu Xu

doi:10.3389/fphar.2020.00021

Characterization and Neuroprotection Potential of Seleno-Polymannuronate

Front Pharmacol. 2020 Feb 20:11:21. doi: 10.3389/fphar.2020.00021. eCollection 2020.

Authors

Decheng Bi¹, Xiaofan Li¹, Tong Li¹, Xiuting Li², Zhijian Lin³, Lijun Yao¹, Hui Li¹, Hong Xu¹, Zhangli Hu¹, Zhenqing Zhang⁴, Qiong Liu¹, Xu Xu¹

Affiliations

¹ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
² Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, China.
³ Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, China.
⁴ College of Pharmaceutical Sciences, Soochow University, Suzhou, China.

Abstract

Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of Aβ_1-42 oligomer in vitro and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect.

Keywords: N2a-sw cells; alginate; anti-apoptosis; neuroprotection; seleno-polymannuronate.