Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets

Methods Mol Biol. 2020:2121:129-140. doi: 10.1007/978-1-0716-0338-3_12.

Abstract

Innate lymphoid cells (ILCs) are important regulators of the early responses to infection at mucosal barriers, including the intestine. Recently, we have shown that specific ILC3 subsets protect against enteric bacterial pathogens. Here, we describe a mouse model of oral infection by Yersinia enterocolitica (Y. enterocolitica) and several different methodologies to assess the severity of the infection. We also detail how ILC3 subsets can be isolated from the mouse small intestine and transferred into recipient immune deficient mice to study the function of these ILCs in the small intestine.

Keywords: Adoptive transfer; Group 3 innate lymphoid cells; Innate immunity; Small intestine lamina propria; Yersinia enterocolitica.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer / methods*
  • Animals
  • Homeodomain Proteins / genetics
  • Immunity, Innate*
  • Intestine, Small / cytology
  • Intestine, Small / immunology*
  • Intestine, Small / microbiology
  • Lymphocyte Subsets / cytology*
  • Lymphocyte Subsets / immunology*
  • Mice
  • Mice, Knockout
  • Mucous Membrane / cytology
  • Mucous Membrane / immunology*
  • Yersinia Infections / immunology*

Substances

  • Homeodomain Proteins
  • RAG-1 protein