Seleno-Chitosan induces apoptosis of lung cancer cell line SPC-A-1 via Fas/FasL pathway

Bioorg Chem. 2020 Apr:97:103701. doi: 10.1016/j.bioorg.2020.103701. Epub 2020 Feb 29.

Abstract

In this study, human lung cancer SPC-A-1 cells were cultured with Seleno-Chitosan to study the mechanism of apoptosis. CCK-8 results showed that with the increase of the concentration of Seleno-Chitosan and the prolongation of culture time, the inhibition on the proliferation of SPC-A-1 cells gradually increased, and the morphology of SPC-A-1 cells changed obviously. Flow cytometry result suggested that the concentration of calcium ion, level of reactive oxygen species and mitochondrial membrane potential increased. Western blot showed that Seleno-Chitosan induced apoptosis via the mitochondrial pathway in SPC-A-1. Eventually, we found 15 proteins that were expressed abnormally by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), which were all related to Fas/FasL pathway of cells.

Keywords: Annexin V; Apoptosis protein; In vitro; Seleno-Chitosan; WB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Chitosan / analogs & derivatives
  • Chitosan / pharmacology*
  • Fas Ligand Protein / metabolism
  • Humans
  • Lung Neoplasms / drug therapy*
  • Lung Neoplasms / metabolism
  • Membrane Potential, Mitochondrial / drug effects
  • Organoselenium Compounds / chemistry
  • Organoselenium Compounds / pharmacology*
  • Signal Transduction / drug effects*
  • fas Receptor / metabolism

Substances

  • Antineoplastic Agents
  • FAS protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Organoselenium Compounds
  • fas Receptor
  • Chitosan