Isolation and identification of a new sildenafil analogue, hydroxycarbodenafil, found as an adulterant in a health supplement

Abstract

During routine screening of illegal adulterants in health supplements, a novel sildenafil analogue was discovered, and subsequently isolated by recrystallization. Its structure was elucidated by extensive analyses of high resolution mass spectrometry (HRMS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) data. The analogue was finally determined as hydroxycarbodenafil, featuring a hydroxyethyl group instead of an ethyl group on piperazine ring in comparison with carbodenafil.

Keywords: Adulterated health supplement; HR-MS and NMR; Hydroxycarbodenafil; Sildenafil analogue.