Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment

Mingxia Zhang; Anzhang Li; Qing Yao; Qingping Wu; Honghui Zhu

doi:10.1016/j.biortech.2019.122626

Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment

Bioresour Technol. 2020 Jun:305:122626. doi: 10.1016/j.biortech.2019.122626. Epub 2019 Dec 16.

Authors

Mingxia Zhang¹, Anzhang Li¹, Qing Yao², Qingping Wu¹, Honghui Zhu³

Affiliations

¹ State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
² College of Horticulture, South China Agricultural University, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, PR China.
³ State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China. Electronic address: zhuhh@gdim.cn.

PMID: 32143020
DOI: 10.1016/j.biortech.2019.122626

Abstract

A heterotrophic nitrifying-aerobic denitrifying bacterium isolated from deep-sea sediment was identified as Pseudomonas bauzanensis DN13-1. Nitrogen (N) removal capability and relative expression of nitrification and denitrification genes of this strain were investigated. The NO₂^--N, NO₃^--N and NH₄⁺-N removal efficiencies were 98.82%, 65.87% and 98.89%, respectively, and strain DN13-1 could efficiently remove mixed N. Meanwhile, other inorganic N was not accumulated during these N removal processes. Genomic analysis indicated that genes nirS, norB, nosZ, nasA and putative amo were identified. The relative expression of functional genes by real-time PCR (qPCR) further confirmed nitrite, nitrate and ammonium removal pathways of strain DN13-1 under aerobic condition. Especially, the ammonium removal pathway of this strain was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification directly. Taken together, strain DN13-1 possesses particularity to efficiently remove N, which guarantees its promising application in aquaculture wastewater treatment.

Keywords: Aerobic denitrification; Heterotrophic nitrification; Nitrogen removal pathway; Pseudomonas bauzanensis.