A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Alisha House; Erica Fatica; Rohan Shah; Jared Stergar; Ryan Pearce; Yana Sandlers

doi:10.1016/j.mex.2019.05.028

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

MethodsX. 2019 May 29:7:100572. doi: 10.1016/j.mex.2019.05.028. eCollection 2020.

Authors

Alisha House¹, Erica Fatica¹, Rohan Shah¹, Jared Stergar¹, Ryan Pearce¹, Yana Sandlers¹

Affiliation

¹ Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States.

Abstract

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

Keywords: GCMS; Induced pluripotent stem cell-derived cardiomyocytes; LC–MS/MS; Metabolic characterization.