Effect of temperature on the growth of Staphylococcus aureus in ready-to-eat cooked rice with pork floss

Kuan-Hung Lu; Yi-Jyun Sheen; Tsui-Ping Huang; Shu-Hui Kao; Chun-Lung Cheng; Cheng-An Hwang; Shiowshuh Sheen; Lihan Huang; Lee-Yan Sheen

doi:10.1016/j.fm.2019.103374

Effect of temperature on the growth of Staphylococcus aureus in ready-to-eat cooked rice with pork floss

Food Microbiol. 2020 Aug:89:103374. doi: 10.1016/j.fm.2019.103374. Epub 2019 Nov 28.

Authors

Kuan-Hung Lu¹, Yi-Jyun Sheen¹, Tsui-Ping Huang², Shu-Hui Kao¹, Chun-Lung Cheng³, Cheng-An Hwang⁴, Shiowshuh Sheen⁴, Lihan Huang⁴, Lee-Yan Sheen⁵

Affiliations

¹ Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
² Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan; Food and Drug Administration, Ministry of Health and Welfare, Taipei 11561, Taiwan.
³ Food Technology and Processing Section, Department of Animal Industry, Council of Agriculture, Taipei 10014, Taiwan.
⁴ Eastern Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.
⁵ Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan; Center for Food and Biomolecules, National Taiwan University, Taipei 10617, Taiwan; National Center for Food Safety Education and Research, National Taiwan University, Taipei 10617, Taiwan. Electronic address: lysheen@ntu.edu.tw.

PMID: 32138980
DOI: 10.1016/j.fm.2019.103374

Abstract

Cooked rice with pork floss (CRPF) wrapped in dried seaweed is one of the most popular ready-to-eat (RTE) foods in many Asian countries, particularly in Taiwan. The products are susceptible to Staphylococcus aureus contamination and temperature abuse during manufacturing, distribution, and storage. The objective of this study was to examine the effect of temperature on its growth in RTE CRPF for use in risk assessment and prevention of staphylococcal food poisoning (SFP). Inoculated CRPF samples were stored at 4, 12, 18, 25, and 35°C, and the change in the populations of S. aureus during storage were analyzed using three primary models to determine specific growth rate (μ_max), lag-phase duration (λ), and maximum population density (y_max). The Ratkowsky square-root and Huang square-root (HSR) models were used as the secondary models to describe the effect of temperature on μ_max, and a linear and an exponential regression models were used to describe the effect of temperature on λ and y_max, respectively. The model performance was evaluated by the root mean square error (RMSE), bias factor (B_f), and accuracy factor (A_f) when appropriate. Results showed that three primary models were suitable for describing the growth curves, with RMSE ≤ 0.3 (log MPN/g). Using μ_max obtained from the Huang model, the minimum growth temperature (T_min) estimated by the HSR model was 7.0°C, well in agreement with the reported T_min. The combination of primary and secondary models for predicting S. aureus growth was validated by additional growth curves at 30°C, which showed that the RMSE was 0.6 (log MPN/g). Therefore, the developed models were acceptable for predicting the growth of S. aureus in CRPF under likely temperature abuse conditions and can be applied to assess the risk of S. aureus in CRPF and design temperature controls to reduce the risk of SFP.

Keywords: IPMP 2013; Predictive microbiology; Rice ball; Staphylococcus aureus.

Publication types

Validation Study

MeSH terms

Animals
Food Handling
Food Safety*
Meat Products / analysis*
Models, Biological
Oryza
Staphylococcus aureus / growth & development*
Swine
Temperature*