L-Lysine-modified Fe3O4 nanoparticles for magnetic cell labeling

Alexander M Demin; Alexander V Mekhaev; Oleg F Kandarakov; Vladimir I Popenko; Olga G Leonova; Aidar M Murzakaev; Dmitry K Kuznetsov; Mikhail A Uimin; Artem S Minin; Vladimir Ya Shur; Alexander V Belyavsky; Victor P Krasnov

doi:10.1016/j.colsurfb.2020.110879

L-Lysine-modified Fe₃O₄ nanoparticles for magnetic cell labeling

Colloids Surf B Biointerfaces. 2020 Jun:190:110879. doi: 10.1016/j.colsurfb.2020.110879. Epub 2020 Feb 19.

Affiliations

¹ Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 22 S. Kovalevskoy St., Yekaterinburg, 620990, Russia. Electronic address: amd2002@mail.ru.
² Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 22 S. Kovalevskoy St., Yekaterinburg, 620990, Russia.
³ Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilova St., Moscow 119991, Russia.
⁴ Institute of Electrophysics, Russian Academy of Sciences (Ural Branch), 106 Amudsen St., Yekaterinburg, 620016, Russia; Institute of Natural Sciences and Mathematics, Ural Federal University, 51 Lenin Ave., Yekaterinburg 620000, Russia.
⁵ Institute of Natural Sciences and Mathematics, Ural Federal University, 51 Lenin Ave., Yekaterinburg 620000, Russia.
⁶ Mikheev Institute of Metal Physics, Russian Academy of Sciences (Ural Branch), 18 S. Kovalevskoy St., Yekaterinburg, 620990, Russia.
⁷ Postovsky Institute of Organic Synthesis, Russian Academy of Sciences (Ural Branch), 22 S. Kovalevskoy St., Yekaterinburg, 620990, Russia; Institute of Chemical Engineering, Ural Federal University, 19 Mira St., Yekaterinburg, 620002, Russia.

PMID: 32135495
DOI: 10.1016/j.colsurfb.2020.110879

Abstract

The efficiency of magnetic labeling with L-Lys-modified Fe₃O₄ magnetic nanoparticles (MNPs) and the stability of magnetization of rat adipose-derived mesenchymal stem cells, lineage-negative (Lin(-)) hematopoietic progenitor cells from mouse bone marrow and human leukemia K562 cells were studied. For this purpose, covalent modification of MNPs with 3-aminopropylsilane and N-di-Fmoc-L-lysine followed by removal of N-protecting groups was carried out. Since the degree of hydroxylation of the surface of the starting nanoparticles plays a crucial role in the silanization reaction and the possibility of obtaining stable colloidal solutions. In present work we for the first time performed a comparative qualitative and quantitative evaluation of the number of adsorbed water molecules and hydroxyl groups on the surface of chemically and physically obtained Fe₃O₄ MNPs using comprehensive FTIR spectroscopy and thermogravimetric analysis. The results obtained can be further used for magnetic labeling of cells in experiments in vitro and in vivo.

Keywords: Hematopoietic; Human leukemia cells; L-Lysine; Magnetic labeling; Magnetic nanoparticles; Mesenchymal stem cells; Progenitor cells.

MeSH terms

Animals
Cells, Cultured
Ferric Compounds / chemistry*
Humans
K562 Cells
Lysine / chemistry*
Magnetic Phenomena
Magnetite Nanoparticles / chemistry*
Particle Size
Rats
Surface Properties

Substances

Ferric Compounds
Magnetite Nanoparticles
ferric oxide
Lysine