Microenergy acoustic pulses induced myogenesis of urethral striated muscle stem/progenitor cells

Kai Cui; Ning Kang; Lia Banie; Tie Zhou; Tianshu Liu; Bohan Wang; Yajun Ruan; Dongyi Peng; Hsun Shuan Wang; Tianyu Wang; Guifang Wang; Amanda B Reed-Maldonado; Zhong Chen; Guiting Lin; Tom F Lue

doi:10.21037/tau.2019.08.18

Microenergy acoustic pulses induced myogenesis of urethral striated muscle stem/progenitor cells

Transl Androl Urol. 2019 Oct;8(5):489-500. doi: 10.21037/tau.2019.08.18.

Authors

Kai Cui^{1

2}, Ning Kang¹, Lia Banie¹, Tie Zhou^{1

3}, Tianshu Liu¹, Bohan Wang¹, Yajun Ruan¹, Dongyi Peng¹, Hsun Shuan Wang¹, Tianyu Wang¹, Guifang Wang¹, Amanda B Reed-Maldonado¹, Zhong Chen^{1

2}, Guiting Lin¹, Tom F Lue¹

Affiliations

¹ Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA.
² Department of Urology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
³ Department of Urology, Shanghai Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.

Abstract

Background: Stress urinary incontinence (SUI) is a common disorder with high prevalence in women across their life span, but there are no non-surgical curative options for the condition. Stem cell-based therapy, especially endogenous stem cell therapy may be a potential treatment method for SUI. The aims of this study are to identify, isolate, and assay the function of urethral striated muscle derived stem/progenitor cells (uMDSCs) and to assess uMDSC response to microenergy acoustic pulses (MAP).

Methods: Urethral striated muscle was identified utilizing 3D imaging of solvent organs (3DISCO) and immunofluorescence (IF). uMDSCs were isolated and purified from Zucker Lean (ZL) (ZUC-LEAN) (ZUC-Leprfa 186) rats, with magnetic-activated cell sorting (MACS) and pre-plating methods. The stemness and differentiation potential of the uMDSCs were measured by cell proliferation, EdU, flow cytometry, IF, and Western blot.

Results: Comparison of the cell proliferation assays between MACS and pre-plating reveals the advantage of MACS over pre-plating. In addition, the study reveals that uMDSCs form myotubes when treated with MAP.

Conclusions: The uMDSCs within female rat urethral striated muscle could be a therapeutic target of MAP in managing SUI.

Keywords: Stress urinary incontinence (SUI); Zucker Lean (ZL) rats; microenergy acoustic pulses (MAP); myogenesis; urethral striated muscle derived stem/progenitor cells (uMDSCs).