Carbon sink strength of nodules but not other organs modulates photosynthesis of faba bean (Vicia faba) grown under elevated [CO2 ] and different water supply

Abstract

Photosynthetic stimulation by elevated [CO₂ ] (e[CO₂ ]) may be limited by the capacity of sink organs to use photosynthates. In many legumes, N₂ -fixing symbionts in root nodules provide an additional sink, so that legumes may be better able to profit from e[CO₂ ]. However, drought not only constrains photosynthesis but also the size and activity of sinks, and little is known about the interaction of e[CO₂ ] and drought on carbon sink strength of nodules and other organs. To compare carbon sink strength, faba bean was grown under ambient (400 ppm) or elevated (700 ppm) atmospheric [CO₂ ] and subjected to well-watered or drought treatments, and then exposed to ¹³ C pulse-labelling using custom-built chambers to track the fate of new photosynthates. Drought decreased ¹³ C uptake and nodule sink strength, and this effect was even greater under e[CO₂ ], and was associated with an accumulation of amino acids in nodules. This resulted in decreased N₂ fixation, and increased accumulation of new photosynthates (¹³ C/sugars) in leaves, which in turn can feed back on photosynthesis. Our study suggests that nodule C sink activity is key to avoid sink limitation in legumes under e[CO₂ ], and legumes may only be able to achieve greater C gain if nodule activity is maintained.

Keywords: 13C allocation; N2 feedback mechanism; climate change; drought; legume; photosynthetic acclimation; root exudates; sink limitation.