The Inhibitory Activity of Picria fel-terrae Lour Herbs Extract on Nitric Oxide Production toward RAW 264.7 Cells Induced by Lipopolysaccharide

Novycha Auliafendri; Rosidah Rosidah; Yuandani Yuandani; Sri Suryani; Denny Satria

doi:10.3889/oamjms.2019.493

The Inhibitory Activity of Picria fel-terrae Lour Herbs Extract on Nitric Oxide Production toward RAW 264.7 Cells Induced by Lipopolysaccharide

Open Access Maced J Med Sci. 2019 Nov 14;7(22):3737-3740. doi: 10.3889/oamjms.2019.493. eCollection 2019 Nov 30.

Authors

Novycha Auliafendri¹, Rosidah Rosidah¹, Yuandani Yuandani¹, Sri Suryani², Denny Satria³

Affiliations

¹ Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
² Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia.
³ Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.

Abstract

Aim: The objective of this study was to evaluate the inhibitory activity of Picria fel-terrae Lour on Nitric Oxide production toward RAW 264.7 cells.

Methods: The extraction was obtained by maceration method using n-hexane, ethyl acetate and ethanol solvents and then nitric oxide (NO) production was obtained using Griess reagent.

Results: Extract of Picria fel-terrae Lour herbs can reduce the NO production toward RAW 264.7 cells with induced by lipopolysaccharide has obtained nitric concentrations 12.5 and 25 μg/mL from n-hexane extract (72.50 ± 4.51 and 10.42 ± 1.82), ethyl acetate extract: (88.33 ± 6.51 and 30.83 ± 6.86), ethanol extract: (75.00 ± 1.91 and 22.08 ± 2.53).

Conclusion: n-hexane extract of Picria fel-terrae Lour Herbs has a high potential to reduce the NO production in LPS-stimulated RAW 264.7 cells compared to ethyl acetate and ethanol extracts of Picria fel-terrae Lour Herbs.

Keywords: Immunosuppressive effects; Nitric Oxide production; Picria fel-terrae Lour Herbs Extract.