Cell density, Lipidomic profile, and fatty acid characterization as selection criteria in bioprospecting of microalgae and cyanobacterium for biodiesel production

Sabarathinam Shanmugam; Thangavel Mathimani; Susaimanickam Anto; M P Sudhakar; Smita S Kumar; Arivalagan Pugazhendhi

doi:10.1016/j.biortech.2020.123061

Cell density, Lipidomic profile, and fatty acid characterization as selection criteria in bioprospecting of microalgae and cyanobacterium for biodiesel production

Bioresour Technol. 2020 May:304:123061. doi: 10.1016/j.biortech.2020.123061. Epub 2020 Feb 20.

Authors

Sabarathinam Shanmugam¹, Thangavel Mathimani², Susaimanickam Anto², M P Sudhakar³, Smita S Kumar⁴, Arivalagan Pugazhendhi⁵

Affiliations

¹ Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
² Department of Energy and Environment, National Institute of Technology, Tiruchirappalli - 620015, Tamil Nadu, India.
³ Marine Biotechnology, National Institute of Ocean Technology, Chennai - 600100, India.
⁴ Department of Environmental Sciences, J.C. Bose University of Science and Technology, YMCA, Mathura Rd, Sector 6, Faridabad, Haryana - 121006, India.
⁵ Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam. Electronic address: arivalagan.pugazhendhi@tdtu.edu.vn.

PMID: 32127245
DOI: 10.1016/j.biortech.2020.123061

Abstract

Selection of indigenous and potential algal strain with high lipid content is paramount challenge in the avenues of microalgal biodiesel production. Particularly, hyper lipid producing algae with maximal triacyglycerols (TAGs) content and preferable fatty acid composition is of interest for sustainable biodiesel. Hence, the present study on comparative assessment of Chlorella vulgaris, Scenedesmus sp. and Synechococcus sp. was done in terms of cell density, lipid, TAGs and fatty acid. Higher biomass yield was obtained in Chlorella vulgaris (0.54 gL^-1) on 13th day while maximal lipid content of 36% was observed in Scenedesmus sp. followed by Chlorella vulgaris (33%). Lipidomic analysis revealed higher non-polar lipids inChlorella vulgaris (57%) and Scenedesmus sp. (54%), whereas in Synechococcus sp. 69% polar lipids were present. In fatty acid profile, C24:0 (22.11%) was predominant in Chlorella vulgaris, while C20:0 (31.72%) and C18:2 (22.26%) was prevalent in Scenedesmus sp. and Synechococcus sp. respectively.

Keywords: Biodiesel; Chlorella; Fatty acid; Lipid; Microalgae; Scenedesmus.

MeSH terms

Biofuels
Biomass
Bioprospecting*
Cell Count
Chlorella vulgaris*
Fatty Acids
Lipidomics
Microalgae*
Patient Selection

Substances

Biofuels
Fatty Acids