We demonstrate the biochip efficacies of three different polymer films (agarose, poly(oligo(ethylene glycol) methacrylate) (pOEGMA), and poly(2-hydroxyethyl metacharylate) (pHEMA) on microscopic glass surfaces. As a result, the non-biofouling performances increased in this order: agarose < pOEGMA < pHEMA, and the binding capabilities increased in this order: pHEMA < pOEGMA < agarose.