Distinct Mechanisms of Dissemination of NDM-1 Metallo-β-Lactamase in Acinetobacter Species in Argentina

Mark D Adams; Fernando Pasteran; German M Traglia; Jasmine Martinez; Fanny Huang; Christine Liu; Jennifer S Fernandez; Carolina Lopez; Lisandro J Gonzalez; Ezequiel Albornoz; Alejandra Corso; Alejandro J Vila; Robert A Bonomo; Maria Soledad Ramirez

doi:10.1128/AAC.00324-20

Distinct Mechanisms of Dissemination of NDM-1 Metallo-β-Lactamase in Acinetobacter Species in Argentina

Antimicrob Agents Chemother. 2020 Apr 21;64(5):e00324-20. doi: 10.1128/AAC.00324-20. Print 2020 Apr 21.

Authors

Mark D Adams¹, Fernando Pasteran², German M Traglia³, Jasmine Martinez⁴, Fanny Huang⁴, Christine Liu⁴, Jennifer S Fernandez⁴, Carolina Lopez⁵, Lisandro J Gonzalez^{5

6}, Ezequiel Albornoz², Alejandra Corso², Alejandro J Vila^{5

6}, Robert A Bonomo^{7

8

9

10

11

12

13}, Maria Soledad Ramirez¹⁴

Affiliations

¹ The Jackson Laboratory, Farmington, Connecticut, USA.
² National/Regional Reference Laboratory for Antimicrobial Resistance (NRL), Servicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina.
³ Departamento de Desarrollo Biotecnología, Instituto de Higiene, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.
⁴ Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California, USA.
⁵ Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, Argentina.
⁶ Área Biofísica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
⁷ Research Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
⁸ Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
⁹ Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹⁰ Department of Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹¹ Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹² Department of Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹³ CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA.
¹⁴ Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, California, USA msramirez@fullerton.edu.

Abstract

A 4-year surveillance of carbapenem-resistant Acinetobacter spp. isolates in Argentina identified 40 strains carrying bla_NDM-1 Genome sequencing revealed that most were Acinetobacter baumannii, whereas seven represented other Acinetobacter spp. The A. baumannii genomes were closely related, suggesting recent spread. bla_NDM-1 was located in the chromosome of A. baumannii strains and on a plasmid in non-A. baumannii strains. A resistance gene island carrying bla_PER-7 and other resistance determinants was found on a plasmid in some A. baumannii strains.

Keywords: Acinetobacter; Acinetobacter baumannii; NDM-1; carbapenem resistance; carbapenems.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acinetobacter Infections / drug therapy
Acinetobacter Infections / microbiology
Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification
Anti-Bacterial Agents / pharmacology*
Argentina
Carbapenems / pharmacology*
Genome, Bacterial / genetics
Humans
Microbial Sensitivity Tests
Plasmids / genetics
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Carbapenems
beta-Lactamases
beta-lactamase NDM-1

Abstract

Publication types

MeSH terms

Substances

Grants and funding