This study focused on supplementing pikeperch (Sander lucioperca) larvae with rotifers fed with Chlorella vulgaris during the first 15 days post hatching (dph). Larvae were fed a combination of rotifers and artemia under three different enrichments: A) Nannochloropsis occulata, B) Chlorella vulgaris, and C) a commercial enrichment-Selco, Spresso from INVE. After 17 days from the trial initiation differences were found between treatments on survival rate, myomere height (MH), fatty acid composition, and stress tolerance. In terms of survival, larvae from treatment b (74.5%) and c (66%) excelled over the control (a) treatment (59%). Furthermore, larvae from both the Chlorella (b) and the Selco (c) treatments showed more resilience to stress conditions (10% and 37% reduction in mortality) when exposed to high salinity conditions (18ppt) for 3 h (stress response). Overall, larvae from treatments b and c performed better than those receiving a non-enriched diet (a), likely due to the higher levels of Essential Fatty Acids (EFA) and the ability of pikeperch to desaturate and elongate fatty acids (FA) with 18 carbons to LC PUFAs (Polyunsaturated Fatty Acids). The present study provides valuable input for designing improved feeding protocols, which will increase the efficiency of pikeperch larval culture.