Retrospective Screening and Analysis of mcr-1 and bla NDM in Gram-Negative Bacteria in China, 2010-2019

Rong Fan; Chuchu Li; Ran Duan; Shuai Qin; Junrong Liang; Meng Xiao; Dongyue Lv; Huaiqi Jing; Xin Wang

doi:10.3389/fmicb.2020.00121

Retrospective Screening and Analysis of mcr-1 and bla _NDM in Gram-Negative Bacteria in China, 2010-2019

Front Microbiol. 2020 Feb 11:11:121. doi: 10.3389/fmicb.2020.00121. eCollection 2020.

Authors

Rong Fan¹, Chuchu Li^{1

2}, Ran Duan¹, Shuai Qin¹, Junrong Liang¹, Meng Xiao¹, Dongyue Lv¹, Huaiqi Jing¹, Xin Wang¹

Affiliations

¹ State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases - National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
² Department of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.

Abstract

Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or bla _NDM genes are increasing. In this study, mcr-1 and bla _NDM distribution of 12,858 Gram-negative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the bla _NDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the bla _NDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or bla _NDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and bla _NDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.

Keywords: Gram-negative; MCR; NDM; carbapenem; polymyxin.