Abstract
An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motif via sortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibodies / chemistry*
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Antibodies / pharmacology
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / pharmacology
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Cell Line, Tumor
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Clathrin / metabolism
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Endocytosis / drug effects
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ErbB Receptors / metabolism
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Humans
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Immunoconjugates / chemistry*
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Immunoconjugates / pharmacology
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Lysosomes / metabolism*
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Molecular Conformation
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Molecular Targeted Therapy
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Optical Imaging
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Peptides / chemistry*
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Peptides / metabolism
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Protein Binding
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Protein Transport / drug effects
Substances
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Antibodies
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Antineoplastic Agents
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Clathrin
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Immunoconjugates
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Peptides
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ErbB Receptors