Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet

Chengbin Xue; Huifang Xie; James Eichenbaum; Yi Chen; Yonggang Wang; Floor W van den Dolder; Junmin Lee; KangJu Lee; Shiming Zhang; Wujin Sun; Amir Sheikhi; Samad Ahadian; Nureddin Ashammakhi; Mehmet R Dokmeci; Han-Jun Kim; Ali Khademhosseini

doi:10.1002/biot.201900456

Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet

Biotechnol J. 2020 Aug;15(8):e1900456. doi: 10.1002/biot.201900456. Epub 2020 Mar 16.

Authors

Chengbin Xue^{1

2

3

4}, Huifang Xie^{1

2

5}, James Eichenbaum^{1

2}, Yi Chen^{1

2

6}, Yonggang Wang^{1

2

7}, Floor W van den Dolder^{1

2

8

9}, Junmin Lee^{1

2}, KangJu Lee^{1

2}, Shiming Zhang^{1

2}, Wujin Sun^{1

2}, Amir Sheikhi^{1

2

10

11}, Samad Ahadian^{1

2}, Nureddin Ashammakhi^{1

2

12}, Mehmet R Dokmeci^{1

2

12}, Han-Jun Kim^{1

2}, Ali Khademhosseini^{1

2

12

13}

Affiliations

¹ Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, Los Angeles, CA, 90095, USA.
² Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
³ Key Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 226001, P. R. China.
⁴ Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, P. R. China.
⁵ Carbohydrate Laboratory, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, P. R. China.
⁶ Beijing Biosis Healing Biological Technology Co., Ltd, Beijing, 102600, P. R. China.
⁷ Guangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
⁸ Division of Heart and Lungs, Department of Cardiothoracic Surgery, University Medical Center Utrecht, Utrecht, GA, 3508, The Netherlands.
⁹ Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, CT, 3584, The Netherlands.
¹⁰ Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
¹¹ Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
¹² Department of Radiological Sciences, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
¹³ Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, CA, 90095, USA.

Abstract

Injectable shear-thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Previously, an iSTB was developed by combining gelatin with synthetic silicate nanoplatelets (SNPs) for potential application to hemostasis and endovascular embolization. Hence, iSTBs are synthesized by varying compositions of gelatin and SNPs to navigate their material, mechanical, rheological, and bioactive properties. All compositions (each component percentage; 1.5-4.5%/total solid ranges; 3-9%) tested are injectable through both 5 Fr general catheter and 2.4 Fr microcatheter by manual pressure. In the results, an increase in gelatin contents causes decrease in swellability, increase in freeze-dried hydrogel scaffold porosity, increase in degradability and injection force during iSTB fabrication. Meanwhile, the amount of SNPs in composite hydrogels is mainly required to decrease degradability and increase shear thinning properties of iSTB. Finally, in vitro and in vivo biocompatibility tests show that the 1.5-4.5% range gelatin-SNP iSTBs are not toxic to the cells and animals. All results demonstrate that the iSTB can be modulated with specific properties for unmet clinical needs. Understanding of mechanical and biological consequences of the changing gelatin-SNP ratios through this study will shed light on the biomedical applications of iSTB on specific diseases.

Keywords: gelatin; injectability; laponite; rheology; shear-thinning biomaterial.

MeSH terms

Animals
Biocompatible Materials* / chemical synthesis
Biocompatible Materials* / toxicity
Cell Line
Cell Survival / drug effects
Embolization, Therapeutic
Gelatin* / chemistry
Hemostasis
Hydrogels
Rheology
Silicates* / chemistry

Substances

Biocompatible Materials
Hydrogels
Silicates
Gelatin

Abstract

MeSH terms

Substances

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