Draft Genome Assemblies of Ionic Liquid-Resistant Yarrowia lipolytica PO1f and Its Superior Evolved Strain, YlCW001

Caleb Walker; Seunghyun Ryu; Sajeet Haridas; Hyunsoo Na; Matthew Zane; Kurt LaButti; Kerrie Barry; Igor V Grigoriev; Cong T Trinh

doi:10.1128/MRA.01356-19

Draft Genome Assemblies of Ionic Liquid-Resistant Yarrowia lipolytica PO1f and Its Superior Evolved Strain, YlCW001

Microbiol Resour Announc. 2020 Feb 27;9(9):e01356-19. doi: 10.1128/MRA.01356-19.

Authors

Caleb Walker¹, Seunghyun Ryu¹, Sajeet Haridas², Hyunsoo Na², Matthew Zane², Kurt LaButti², Kerrie Barry², Igor V Grigoriev², Cong T Trinh³

Affiliations

¹ Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA.
² U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, USA.
³ Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, Tennessee, USA ctrinh@utk.edu.

Abstract

Adaptive laboratory evolution of Yarrowia lipolytica PO1f in the benchmark ionic liquid (IL; 1-ethyl-3-methylimidazolium acetate) produced a superior IL-tolerant microorganism, strain YlCW001. Here, we report the genome sequences of PO1f and YlCW001 to study the robustness of Y. lipolytica and its potential use as a microbial platform for producing fuels and chemicals.