Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Jixiang Zhu; Xingwu Zhou; Han-Jun Kim; Moyuan Qu; Xing Jiang; KangJu Lee; Li Ren; Qingzhi Wu; Canran Wang; Xunmin Zhu; Peyton Tebon; Shiming Zhang; Junmin Lee; Nureddin Ashammakhi; Samad Ahadian; Mehmet Remzi Dokmeci; Zhen Gu; Wujin Sun; Ali Khademhosseini

doi:10.1002/smll.201905910

Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Small. 2020 Apr;16(16):e1905910. doi: 10.1002/smll.201905910. Epub 2020 Feb 26.

Authors

Jixiang Zhu^{1

2

3}, Xingwu Zhou¹, Han-Jun Kim¹, Moyuan Qu^{1

4}, Xing Jiang^{1

5}, KangJu Lee¹, Li Ren¹, Qingzhi Wu¹, Canran Wang¹, Xunmin Zhu^{2

3}, Peyton Tebon¹, Shiming Zhang¹, Junmin Lee¹, Nureddin Ashammakhi^{1

6

7}, Samad Ahadian¹, Mehmet Remzi Dokmeci^{1

6}, Zhen Gu^{1

8

9}, Wujin Sun¹, Ali Khademhosseini^{1

6

8

9

7}

Affiliations

¹ Department of Bioengineering, Center for Minimally Invasive Therapeutics, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
² Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436, China.
³ Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
⁴ State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
⁵ School of Nursing, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
⁶ Department of Radiological Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
⁷ Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
⁸ Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
⁹ California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Abstract

The extraction of interstitial fluid (ISF) from skin using microneedles (MNs) has attracted growing interest in recent years due to its potential for minimally invasive diagnostics and biosensors. ISF collection by absorption into a hydrogel MN patch is a promising way that requires the materials to have outstanding swelling ability. Here, a gelatin methacryloyl (GelMA) patch is developed with an 11 × 11 array of MNs for minimally invasive sampling of ISF. The properties of the patch can be tuned by altering the concentration of the GelMA prepolymer and the crosslinking time; patches are created with swelling ratios between 293% and 423% and compressive moduli between 3.34 MPa and 7.23 MPa. The optimized GelMA MN patch demonstrates efficient extraction of ISF. Furthermore, it efficiently and quantitatively detects glucose and vancomycin in ISF in an in vivo study. This minimally invasive approach of extracting ISF with a GelMA MN patch has the potential to complement blood sampling for the monitoring of target molecules from patients.

Keywords: gelatin methacryloyl; microneedle patches; minimally invasive diagnostics; skin interstitial fluid; transdermal extraction.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Extracellular Fluid*
Gelatin*
Humans
Hydrogels*
Needles / classification*
Skin*

Substances

Hydrogels
Gelatin

Abstract

Publication types

MeSH terms

Substances

Grants and funding