Draft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum

Mol Plant Microbe Interact. 2020 May;33(5):724-726. doi: 10.1094/MPMI-12-19-0356-A. Epub 2020 Mar 24.

Abstract

Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen.

Keywords: Macrophomina phaseolina; MinION; Oxford Nanopore; charcoal rot; sorghum.

MeSH terms

  • Ascomycota / genetics*
  • Edible Grain / microbiology
  • Genome, Fungal*
  • Plant Diseases / microbiology*
  • Sorghum / microbiology*