An assay for Staphylococcus aureus based on a self-catalytic ampicillin-metal (Fe3+)-organic gels-H2O2 chemiluminescence system with near-zero background noise

Chem Commun (Camb). 2020 Mar 21;56(23):3421-3424. doi: 10.1039/c9cc09166a. Epub 2020 Feb 25.

Abstract

A self-catalytic ampicillin-metal (Fe3+)-organic gels (AMP-MOGs (Fe))-H2O2 CL system, which is not influenced by transition metal ions, was studied. A method for CL detection of Staphylococcus aureus (S. aureus) based on the AMP-MOGs (Fe)-H2O2 CL system was achieved. A superior detection limit of 31 CFU mL-1 toward S. aureus was obtained with near-zero background noise.

MeSH terms

  • Ampicillin / chemistry*
  • Antibodies / immunology
  • Catalysis
  • Gels / chemistry*
  • Hydrogen Peroxide / chemistry*
  • Immunoassay / methods
  • Iron / chemistry*
  • Limit of Detection
  • Luminescence
  • Luminescent Measurements / methods
  • Phenanthrolines / chemistry
  • Staphylococcus aureus / immunology
  • Staphylococcus aureus / isolation & purification*

Substances

  • 1,10-phenanthroline-2,9-dicarboxylic acid
  • Antibodies
  • Gels
  • Phenanthrolines
  • Ampicillin
  • Hydrogen Peroxide
  • Iron