Heme peroxidase-Trapping intermediates by cryo neutron crystallography

Abstract

By combining the normal practice for X-ray crystallography of collecting diffraction data at 100K with neutron crystallography the structures of cryo-trapped enzyme intermediates have been determined, revealing the positions of the previously hidden hydrogens that are essential to a better understanding of the involved mechanism.

Keywords: Cryo-trapping; Enzyme intermediate; Heme peroxidase; Neutron diffraction; Protonation state.