Cinnamomum verum Bark Extract Mediated Green Synthesis of ZnO Nanoparticles and Their Antibacterial Potentiality

Mohammad Azam Ansari; Mahadevamurthy Murali; Daruka Prasad; Mohammad A Alzohairy; Ahmad Almatroudi; Mohammad N Alomary; Arakere Chunchegowda Udayashankar; Sudarshana Brijesh Singh; Sarah Mousa Maadi Asiri; Bagepalli Shivaram Ashwini; Hittanahallikoppal Gajendramurthy Gowtham; Nataraj Kalegowda; Kestur Nagaraj Amruthesh; Thimappa Ramachandrappa Lakshmeesha; Siddapura Ramachandrappa Niranjana

doi:10.3390/biom10020336

Cinnamomum verum Bark Extract Mediated Green Synthesis of ZnO Nanoparticles and Their Antibacterial Potentiality

Biomolecules. 2020 Feb 19;10(2):336. doi: 10.3390/biom10020336.

Authors

Mohammad Azam Ansari¹, Mahadevamurthy Murali², Daruka Prasad³, Mohammad A Alzohairy⁴, Ahmad Almatroudi⁴, Mohammad N Alomary⁵, Arakere Chunchegowda Udayashankar⁶, Sudarshana Brijesh Singh⁶, Sarah Mousa Maadi Asiri⁷, Bagepalli Shivaram Ashwini⁸, Hittanahallikoppal Gajendramurthy Gowtham², Nataraj Kalegowda², Kestur Nagaraj Amruthesh², Thimappa Ramachandrappa Lakshmeesha^{6

9}, Siddapura Ramachandrappa Niranjana⁶

Affiliations

¹ Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
² Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
³ Department of Physics, B.M.S. Institute of Technology, Bangalore 560 064, India.
⁴ Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia.
⁵ National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh, Saudi Arabia.
⁶ Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru- 570 006, Karnataka, India.
⁷ Department of Biophysics, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
⁸ Department of Microbiology, Sri Siddhartha Medical College, Tumkuru - 572107, Karnataka, India.
⁹ Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bangalore 560056, India.

Abstract

Cinnamomum verum plant extract mediated propellant chemistry route was used for the green synthesis of zinc oxide nanoparticles. Prepared samples were confirmed for their nano regime using advanced characterization techniques such as powder X-ray diffraction and microscopic techniques such as scanning electron microscopy and transmission electron microscopy. The energy band gap of the green synthesized zinc oxide (ZnO)-nanoparticles (NPs) were found between 3.25-3.28 eV. Fourier transmission infrared spectroscopy shows the presence of Zn-O bond within the wave number of 500 cm^-1. SEM images show the specific agglomeration of particles which was also confirmed by TEM studies. The green synthesized ZnO-NPs inhibited the growth of Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 125 µg mL^-1 and 62.5 µg mL^-1, respectively. The results indicate the prepared ZnO-NPs can be used as a potential antimicrobial agent against harmful pathogens.

Keywords: Cinnamomum verum; GC-MS; ZnO-NPs; antibacterial activity; green synthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemical synthesis
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology*
Cinnamomum zeylanicum / chemistry*
Escherichia coli / drug effects
Escherichia coli Infections / drug therapy
Green Chemistry Technology
Humans
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Plant Bark / chemistry
Plant Extracts / chemistry
Staphylococcal Infections / drug therapy
Staphylococcus aureus / drug effects
Zinc Oxide / chemical synthesis
Zinc Oxide / chemistry*
Zinc Oxide / pharmacology*

Substances

Anti-Bacterial Agents
Plant Extracts
Zinc Oxide