NTF-RINT, a new method for the epidemiological surveillance of MDR Mycobacterium tuberculosis L2/Beijing strains

Bernice J Klotoe; Natalia Kurepina; Elena Zholdibayeva; S Panaiotov; Barry N Kreiswirth; Richard Anthony; Christophe Sola; Guislaine Refrégier

doi:10.1016/j.tube.2019.101894

NTF-RINT, a new method for the epidemiological surveillance of MDR Mycobacterium tuberculosis L2/Beijing strains

Tuberculosis (Edinb). 2020 Jan:120:101894. doi: 10.1016/j.tube.2019.101894. Epub 2019 Dec 9.

Authors

Bernice J Klotoe¹, Natalia Kurepina², Elena Zholdibayeva³, S Panaiotov⁴, Barry N Kreiswirth², Richard Anthony⁵, Christophe Sola¹, Guislaine Refrégier⁶

Affiliations

¹ Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
² Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USA.
³ National Center for Biotechnology, Astana, Kazakhstan.
⁴ National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
⁵ RIVM Bilthoven, the Netherlands.
⁶ Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France. Electronic address: guislaine.refregier@u-psud.fr.

PMID: 32090855
DOI: 10.1016/j.tube.2019.101894

Abstract

The most widely discussed antibiotic-resistant tuberculosis strains ("W" and "B0/W148", "CAO") belong to L2/Beijing Lineage and are characterized by IS6110 insertion sequences at the NTF locus. We present a high-throughput, microbead-based method, called NTF-RINT for detection of IS in NTF and Rifampicin and Isoniazid Typing. This method provides tuberculosis diagnostic confirmation, screens for the so-called modern L2/Beijing sublineage and detects mutations involved in resistance to Rifampicin (RIF) and Isoniazid (INH).

Keywords: Atypical Beijing; MDR-TB; Molecular epidemiology; Typical Beijing.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques*
DNA Mutational Analysis
DNA Transposable Elements*
DNA, Bacterial / genetics*
Drug Resistance, Multiple, Bacterial / genetics*
Genotype
High-Throughput Nucleotide Sequencing*
Humans
Kazakhstan / epidemiology
Molecular Epidemiology
Mutation*
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / pathogenicity
New York City / epidemiology
Phenotype
Polymerase Chain Reaction
Population Surveillance
Predictive Value of Tests
Reproducibility of Results
Tuberculosis, Multidrug-Resistant / diagnosis*
Tuberculosis, Multidrug-Resistant / epidemiology
Tuberculosis, Multidrug-Resistant / genetics
Virulence

Substances

DNA Transposable Elements
DNA, Bacterial