Correlative Brillouin and Raman spectroscopy data acquired on single cells

Silvia Caponi; Sara Mattana; Maurizio Mattarelli; Martina Alunni Cardinali; Lorena Urbanelli; Krizia Sagini; Carla Emiliani; Daniele Fioretto

doi:10.1016/j.dib.2020.105223

Correlative Brillouin and Raman spectroscopy data acquired on single cells

Data Brief. 2020 Feb 5:29:105223. doi: 10.1016/j.dib.2020.105223. eCollection 2020 Apr.

Authors

Silvia Caponi¹, Sara Mattana², Maurizio Mattarelli³, Martina Alunni Cardinali³, Lorena Urbanelli⁴, Krizia Sagini⁴, Carla Emiliani⁴, Daniele Fioretto³

Affiliations

¹ Istituto Officina dei Materiali del CNR (CNR-IOM)-Unità di Perugia, University of Perugia, Perugia, I-06123, Italy.
² Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy.
³ Department of Physics and Geology, University of Perugia, Perugia, I-06123, Italy.
⁴ Department of Chemistry, Laboratory of Biochemistry and Molecular Biology, Biology and Biotechnology, University of Perugia, Via del Giochetto, Perugia, I-06123, Italy.

Abstract

The distribution of chemical species and the mechanical modulation inside a single cell or tissue are of fundamental importance to characterize their physiological activity or their pathological conditions [1-4]. Here we analyse these properties by means of label free, non invasive, spectroscopic methods. In particular, we use a recently developed micro-spectrometer, which acquires simultaneously Raman and Brillouin spectra on the same point with subcellular resolution [5]. The techniques ability to analyse the chemical composition and the mechanical properties of single cells has been tested on NIH/3T3 murine fibroblast cells grown in adhesion on silicon substrates. Here we report the data acquired from fixed cells after their oncogenic transformation. Mechanical and chemical evolution is evident by direct inspection of raw data. Sharing our experimental records can be valuable for researchers interested in the analysis of single cells by Raman and Brillouin spectroscopy in order: i) to compare data acquired by different set-ups and ii) to correctly model the fitting functions.

Keywords: Biophotonics; Brillouin spectroscopy; Cell mechanics; Raman spectroscopy.