Real-time cell analysis system in cytotoxicity applications: Usefulness and comparison with tetrazolium salt assays

Justyna Stefanowicz-Hajduk; J Renata Ochocka

doi:10.1016/j.toxrep.2020.02.002

Real-time cell analysis system in cytotoxicity applications: Usefulness and comparison with tetrazolium salt assays

Toxicol Rep. 2020 Feb 7:7:335-344. doi: 10.1016/j.toxrep.2020.02.002. eCollection 2020.

Authors

Justyna Stefanowicz-Hajduk¹, J Renata Ochocka¹

Affiliation

¹ Department of Biology and Pharmaceutical Botany, Medical University of Gdańsk, Al. Hallera 107, 80-416, Gdańsk, Poland.

Abstract

Real-time cell analysis (RTCA) is a technique based on impedance and microsensor electrodes. RTCA system allows label-free, real-time, and continuous monitoring of cell adhesion, morphology, and rate of cell proliferation. The system offers a wide range of applications, mainly in toxicological studies, new drug screening, and microbiology. Here, we describe the usefulness of the system in different applications and compare this technology with conventional endpoint assays based on tetrazolium salts. We present advantages and disadvantages of the system and endpoint methods and their limitations in cytotoxicity investigations.

Keywords: Cell index; Impedance; Microsensor electrodes; RTCA; Tetrazolium salts.

Publication types

Review