High Glucose Exacerbates TNF- α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Wenjun Zhu; Qihong Qiu; Haoyuan Luo; Fuping Zhang; Juan Wu; Xiaorui Zhu; Min Liang

doi:10.1155/2020/4910767

High Glucose Exacerbates TNF- α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Stem Cells Int. 2020 Feb 5:2020:4910767. doi: 10.1155/2020/4910767. eCollection 2020.

Authors

Wenjun Zhu^{1

2}, Qihong Qiu^{1

2}, Haoyuan Luo^{1

2}, Fuping Zhang^{1

2}, Juan Wu^{2

3}, Xiaorui Zhu^{1

2}, Min Liang^{1

2}

Affiliations

¹ Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
² Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
³ Department of Stomatology, Shenzhen Nanshan Hospital, Shenzhen 518000, China.

Abstract

Objective: This research is aimed at investigating how high glucose affects the proliferation and apoptosis in periodontal ligament stem cells (PDLSCs) in the presence of TNF-α.

Methods: PDLSCs obtained from periodontal healthy permanent teeth were treated under either high-glucose condition (30 mmol/L, G30 group) or normal glucose condition (5.6 mmol/L, G5.6 group) in the presence or absence of TNF-α. α. α.

Results: CCK-8 assay showed that high glucose exacerbated TNF-α. α. α. α. α. α.

Conclusion: High glucose exacerbates TNF-α-induced proliferative inhibition in human periodontal ligament stem cells through the upregulation and activation of TNF receptor 1. Inhibition of intracellular ROS expression by vitamin C partially rescues PDLSCs in terms of cell proliferation.α.