Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

Methods Mol Biol. 2020:2105:251-260. doi: 10.1007/978-1-0716-0243-0_16.

Abstract

Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.

Keywords: Biodistribution; IVIS; Tissue accumulation; Whole-body in vivo bioimaging.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Data Analysis
  • Fluorescent Dyes*
  • Imaging, Three-Dimensional
  • Microscopy, Fluorescence
  • Optical Imaging*
  • Peptide Nucleic Acids* / chemical synthesis
  • Peptide Nucleic Acids* / chemistry
  • Spectroscopy, Near-Infrared*
  • Whole Body Imaging*

Substances

  • Fluorescent Dyes
  • Peptide Nucleic Acids