Cellular changes of microbial consortium GY1 during decabromodiphenyl ether (BDE-209) biodegradation and identification of strains responsible for BDE-209 degradation in GY1

Yuanyuan Yu; Hua Yin; Wantang Huang; Hui Peng; Guining Lu; Zhi Dang

doi:10.1016/j.chemosphere.2020.126205

Cellular changes of microbial consortium GY1 during decabromodiphenyl ether (BDE-209) biodegradation and identification of strains responsible for BDE-209 degradation in GY1

Chemosphere. 2020 Jun:249:126205. doi: 10.1016/j.chemosphere.2020.126205. Epub 2020 Feb 13.

Authors

Yuanyuan Yu¹, Hua Yin², Wantang Huang¹, Hui Peng³, Guining Lu¹, Zhi Dang¹

Affiliations

¹ Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou, 510006, Guangdong, China.
² Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou, 510006, Guangdong, China. Electronic address: huayin@scut.edu.cn.
³ Department of Chemistry, Jinan University, Guangzhou, 510632, Guangdong, China.

PMID: 32086068
DOI: 10.1016/j.chemosphere.2020.126205

Abstract

Microbial consortium remediation has been considered to be a promising technique for BDE-209 elimination in water, soil and sediment. Herein, we studied malondialdehyde (MDA), membrane potential (MP), and reactive active species (ROS) of a microbial consortium GY1 exposed to BDE-209. The results indicated that the microbial antioxidant defense system was vulnerable by BDE-209. Both early and late apoptosis of microbial consortium induced by BDE-209 were observed. The sequencing results revealed that Stenotrophomonas, Microbacterium and Sphingobacterium in GY1 played major roles in BDE-209 degradation. Moreover, a novel facultative anaerobic BDE-209 degrading strain named Microbacterium Y2 was identified from GY1, by which approximately 56.1% of 1 mg/L BDE-209 was degraded within 7 days, and intracellular enzymes of which contributed great to the result. Overall, the current study provided new insights to deeply understand the mechanisms of BDE-209 degradation by microbial consortium.

Keywords: Apoptosis; Decabromodiphenyl ether; Microbacterium; Microbial consortium；; Oxidative stress.

MeSH terms

Antioxidants / metabolism
Biodegradation, Environmental
Halogenated Diphenyl Ethers / metabolism
Halogenated Diphenyl Ethers / toxicity*
Malondialdehyde / metabolism
Microbial Consortia / drug effects*
Soil

Substances

Antioxidants
Halogenated Diphenyl Ethers
Soil
Malondialdehyde
decabromobiphenyl ether