Polarization of Macrophages in Epidural Inflammation Induced by Canine Intervertebral Disc Herniation

Núria Vizcaíno Revés; Helga Maria Mogel; Michael Stoffel; Artur Summerfield; Franck Forterre

doi:10.3389/fvets.2020.00032

Polarization of Macrophages in Epidural Inflammation Induced by Canine Intervertebral Disc Herniation

Front Vet Sci. 2020 Jan 31:7:32. doi: 10.3389/fvets.2020.00032. eCollection 2020.

Authors

Núria Vizcaíno Revés¹, Helga Maria Mogel², Michael Stoffel², Artur Summerfield³, Franck Forterre¹

Affiliations

¹ Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
² Division of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
³ Institut für Virologie und Immunologie, Institut für Infektionskrankheiten und Pathobiologie, Universität Bern, Bern, Switzerland.

Abstract

Introduction: Canine interverterbral disc (IVD), although physiologically acellular, displays an inflammatory cell population consisting almost exclusively of macrophages (Mϕ) when acutely herniated. Mϕ encompass a heterogenous cell population, roughly divided into classically (M1) or alternatively activated (M2)Mϕ. Polarization into M1 Mϕ leads to strong antimicrobial activity and pro-inflammatory response. In contrast, M2Mϕ exibit anti-inflammatory function and regulate wound healing. The purpose of this study was to characterize the phenotype of the Mϕ population present in naturally occurring IVD herniation. Materials and Methods: IVD material of dogs with IVD disease was collected during standard decompressive surgery. A negative control consisting of IVD material of dogs without IVD degeneration and a positive control consisting of canine liver and lymph node samples were also included. All samples were embedded in OCT and shock frozen. Eight micrometer cryostat sections were prepared, air dried and immunostained without prefixation or permeabilization. CD14 was used as marker Mϕ, MHCII for M1Mϕ and CD206 for M2Mϕ. Results: Fifteen samples of dogs with IVD herniation, 10 negative, and 5 positive control samples were obtained. No positive cell was found in the negative control group. The positive control group displayed several MHCII and CD206 positive cells, all of them being simultaneously positive to CD14. All herniated samples displayed a mixed population of M1Mϕ and M2Mϕ, and some sparse Mϕ displaying markers for both M1 and M2Mϕ simultaneously. Conclusion: The mixed phenotype encountered shows the plasticity and dynamism of Mϕ and evidences the chronic component of IVD disease despite its acute clinical presentation.

Keywords: canine; disc degeneration; intervertebral disc; macrophages; polarization.