Mapping of QTL and identification of candidate genes conferring spontaneous haploid genome doubling in maize (Zea mays L.)

Jiaojiao Ren; Nicholas A Boerman; Ruixiang Liu; Penghao Wu; Benjamin Trampe; Kimberly Vanous; Ursula K Frei; Shaojiang Chen; Thomas Lübberstedt

doi:10.1016/j.plantsci.2019.110337

Mapping of QTL and identification of candidate genes conferring spontaneous haploid genome doubling in maize (Zea mays L.)

Plant Sci. 2020 Apr:293:110337. doi: 10.1016/j.plantsci.2019.110337. Epub 2019 Nov 21.

Authors

Jiaojiao Ren¹, Nicholas A Boerman², Ruixiang Liu³, Penghao Wu¹, Benjamin Trampe², Kimberly Vanous², Ursula K Frei², Shaojiang Chen⁴, Thomas Lübberstedt⁵

Affiliations

¹ College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052, China.
² Department of Agronomy, Iowa State University, Ames, Iowa, 50011, USA.
³ Institute of Food Crops, Jiangsu Province Academy of Agricultural Sciences, Jiangsu, 210014, China.
⁴ National Maize Improvement Center, China Agricultural University, Beijing, 100193, China.
⁵ Department of Agronomy, Iowa State University, Ames, Iowa, 50011, USA. Electronic address: thomasl@iastate.edu.

PMID: 32081276
DOI: 10.1016/j.plantsci.2019.110337

Abstract

In vivo doubled haploid (DH) technology is widely used in commercial maize (Zea mays L.) breeding. Haploid genome doubling is a critical step in DH breeding. In this study, inbred lines GF1 (0.65), GF3(0.29), and GF5 (0) with high, moderate, and poor spontaneous haploid genome doubling (SHGD), respectively, were selected to develop mapping populations for SHGD. Three QTL, qshgd1, qshgd2, and qshgd3, related to SHGD were identified by selective genotyping. With the exception of qshgd3, the source of haploid genome doubling alleles were derived from GF1. Furthermore, RNA-Seq was conducted to identify putative candidate genes between GF1 and GF5 within the qshgd1 region. A differentially expressed formin-like protein 5 transcript was identified within the qshgd1 region.

Keywords: Doubled haploid (DH); Maize; RNA-seq; Spontaneous haploid genome doubling (SHGD).

MeSH terms

Alleles
Base Sequence
Breeding
Chromosome Mapping
Down-Regulation
Formins / genetics
Genes, Plant / genetics*
Genome, Plant
Genotype
Haploidy*
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait Loci / genetics*
Sequence Analysis, RNA
Zea mays / genetics*

Substances

Formins