For the last two decades there has been wide ranging debate about the status of macroautophagy during mitosis. Because metazoan cells undergo an "open" mitosis in which the nuclear envelope breaks down, it has been proposed that macroautophagy must be inhibited to maintain genome integrity. While many studies have agreed that the number of autophagosomes is greatly reduced in cells undergoing mitosis, there has been no consensus on whether this reflects decreased autophagosome synthesis or increased autophagosome degradation. Reviewing the literature we were concerned that many studies relied too heavily on autophagy assays that were simply not appropriate for a relatively brief event such as mitosis. Using highly dynamic omegasome markers we have recently shown unequivocally that autophagosome synthesis is repressed at the onset of mitosis and is restored once cell division is complete. This is accomplished by CDK1, the master regulator of mitosis, taking over the function of MTORC1, to ensure autophagy is repressed during mitosis.
Keywords: ATG13 (autophagy related 13); Autophagy; CDK1 (cyclin dependent kinase 1); MTOR (mechanistic target of rapamycin kinase); MTORC1 (MTOR complex 1); RPTOR/RAPTOR (regulatory associated protein of MTOR complex 1); ULK1 (unc-51 like autophagy activating kinase 1); mitosis; omegasome.