Target Of Rapamycin pathway in the white-rot fungus Phanerochaete chrysosporium

PLoS One. 2020 Feb 20;15(2):e0224776. doi: 10.1371/journal.pone.0224776. eCollection 2020.

Abstract

The Target Of Rapamycin (TOR) signaling pathway is known to regulate growth in response to nutrient availability and stress in eukaryotic cells. In the present study, we have investigated the TOR pathway in the white-rot fungus Phanerochaete chrysosporium. Inhibition of TOR activity by rapamycin affects conidia germination and hyphal growth highlighting the conserved mechanism of susceptibility to rapamycin. Interestingly, the secreted protein content is also affected by the rapamycin treatment. Finally, homologs of the components of TOR pathway can be identified in P. chrysosporium. Altogether, those results indicate that the TOR pathway of P. chrysosporium plays a central role in this fungus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Fungal Proteins / antagonists & inhibitors
  • Fungal Proteins / metabolism*
  • Humans
  • Hydrogen Bonding
  • Phanerochaete / growth & development*
  • Phanerochaete / metabolism*
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • Proteome
  • Signal Transduction / drug effects
  • Sirolimus / pharmacology
  • Spores, Fungal / metabolism
  • TOR Serine-Threonine Kinases / antagonists & inhibitors
  • TOR Serine-Threonine Kinases / metabolism*
  • beta-Glucosidase / metabolism

Substances

  • Fungal Proteins
  • Proteome
  • TOR Serine-Threonine Kinases
  • beta-Glucosidase
  • Sirolimus

Grants and funding

This work was supported by a grant overseen by the French National Research Agency (ANR) as part of the "Investissements d'Avenir" program (ANR-11-LABX-0002-01, Lab of Excellence ARBRE). DVN was supported by a Doctoral Fellowship from the Ministry of Agriculture and Rural Development, Vietnam (Agricultural and Fisheries Biotechnology Program) and support from French ministry of foreign affair (program Campus France). AFG was supported by a postdoctoral grant from Region Grand Est. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.