Multidrug-Resistant Escherichia coli Strain Isolated from Swine in China Harbors mcr-3.1 on a Plasmid of the IncX1 Type That Cotransfers with mcr-1.1

Dewei Sun; Shanshan Jin; Jing Wang; Zhenyu Wang; Jiajian Fan; Zhengzhong Xu; Yaohui Xu; Xiang Chen; Xin'an Jiao

doi:10.1089/fpd.2019.2769

Multidrug-Resistant Escherichia coli Strain Isolated from Swine in China Harbors mcr-3.1 on a Plasmid of the IncX1 Type That Cotransfers with mcr-1.1

Foodborne Pathog Dis. 2020 Oct;17(10):597-601. doi: 10.1089/fpd.2019.2769. Epub 2020 Feb 20.

Authors

Dewei Sun^{1

2}, Shanshan Jin^{1

2}, Jing Wang^{1

2}, Zhenyu Wang^{1

2}, Jiajian Fan^{1

2}, Zhengzhong Xu^{1

2}, Yaohui Xu³, Xiang Chen^{1

2}, Xin'an Jiao^{1

2}

Affiliations

¹ Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
² Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou, China.
³ College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Henan, China.

PMID: 32077761
DOI: 10.1089/fpd.2019.2769

Abstract

An Escherichia coli strain isolated from the feces of swine at a pork slaughterhouse in Henan province China was found to possess two colistin-resistance genes, mcr-1 and mcr-3, plus 16 additional resistance genes. Genes mcr-1.1 and mcr-3.1 were identified on IncHI2 and IncX1 type plasmids, respectively. Transconjugants (containing mcr-3, mcr-1&mcr-3) were obtained that were 64- and 512-fold higher than the minimum inhibitory concentration of colistin on the recipient bacteria (E. coli C600), respectively. The IncX1 plasmid containing mcr-3.1 displayed a very specific structure compared with previous mcr-3. Variable and stable regions were similar across different plasmids, multiple insertion sequences and transposases.

Keywords: InX1 plasmid; colistin; mcr-1.1; mcr-3.1; multidrug resistance; swine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology*
China
Colistin / pharmacology
DNA, Bacterial
Drug Resistance, Multiple, Bacterial*
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli / metabolism
Escherichia coli Infections / microbiology
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Feces / microbiology
Interspersed Repetitive Sequences
Microbial Sensitivity Tests
Plasmids
Swine
Transferases (Other Substituted Phosphate Groups) / genetics*
Transferases (Other Substituted Phosphate Groups) / metabolism

Substances

Anti-Bacterial Agents
DNA, Bacterial
Escherichia coli Proteins
MCR-1 protein, E coli
MCR-3 protein, E coli
Transferases (Other Substituted Phosphate Groups)
Colistin