The situation of prevention of non-neonatal tetanus in China is severe. Strengthening the active immunization with tetanus toxoid vaccine (TTCV) is the key to prevent the non-neonatal tetanus. Through the detection of tetanus antibody (TAB), the immune status of individual can be determined, so as to implement the active immunization of TTCV correctly. The research on TAB detection technology is stagnant in aboard, but still in a development process in China since there is a realistic demand for TAB detection. This review collects relatively limited data of TAB detection technology in China, and summarizes the techniques such as mice toxin neutralization test (MTNT), indirect hemagglutination assay (IHA), double agar gel immune diffusion test (Rubin method), enzyme-linked immunosorbent assay (ELISA) and colloidal gold (CG), in order to provide a comprehensive basis for domestic TAB detection. The TAB detection technology in China has not yet achieved international recognition due to the lack of comparative study of domestic and international institutions and reference reagents. The special domestic situation of tetanus prevention makes the research of TAB detection technology have a certain practical significance, and rapid detection reagents such as ELISA and CG method have a certain application value in China.
我国非新生儿破伤风防控形势严峻，强化含破伤风类毒素疫苗（TTCV）的主动免疫是非新生儿破伤风防控的关键。通过破伤风抗体（TAB）检测可以明确个体的TTCV免疫状态，从而正确实施TTCV主动免疫。国外对TAB检测技术的研究呈现停滞状态，而国内有现实的TAB检测需求，破伤风抗体检测技术仍然处于动态发展过程中。本文收集国内也相对有限的TAB检测技术资料，对国内应用和发展的小鼠毒素中和试验（MTNT）、间接血凝法（IHA）、凝胶双向扩散试验或Rubin法、酶联免疫吸附试验（ELISA）、胶体金（CG）法等TAB检测技术进行总结，以期为国内TAB检测提供较全面的依据。由于缺乏国内TAB检测和国际机构、参考试剂的对比研究，我国的TAB检测技术尚未实现国际化认可。我国特殊的破伤风防控形势使得TAB检测技术研究在国内有一定的现实意义，ELISA、CG法等快速检测试剂具备一定的应用价值。.
Keywords: Antibody; Colloidal gold; Enzyme-linked immunosorbent assay; Tetanus toxoid; Vaccine.