Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production

James D Budge; Tanya J Knight; Jane Povey; Joanne Roobol; Ian R Brown; Gurdeep Singh; Andrew Dean; Sarah Turner; Colin M Jaques; Robert J Young; Andrew J Racher; C Mark Smales

doi:10.1016/j.dib.2020.105217

Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production

Data Brief. 2020 Jan 31:29:105217. doi: 10.1016/j.dib.2020.105217. eCollection 2020 Apr.

Authors

Affiliations

¹ Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
² Lonza Biologics, 228 Bath Road, Slough, SL1 4DX, UK.
³ Cell Engineering Group, Lonza Biologics, Granta Park, Cambridge, CB21 6GS, UK.

Abstract

The data presented in this article relates to the manuscript entitled 'Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production', published in the Journal Metabolic Engineering [1]. In the article here, we present data examining the overexpression of the lipid metabolism modifying genes SCD1 and SREBF1 in CHO cells by densitometry of western blots and by using mass spectrometry to investigate the impact on specific lipid species. We also present immunofluorescence data at the protein level upon SCD1 and SREBF1 overexpression. The growth profile data during batch culture of control CHO cells and CHO cells engineered to overexpress SCD1 and SREBF1 during batch culture are also reported. Finally, we report data on the yields of model secretory recombinant proteins produced from control, SCD1 or SREBF1 engineered cells using a transient expression systems.

Keywords: Cell line engineering; Chinese hamster ovary (CHO) cells; Difficult to express protein; Endoplasmic reticulum; Lipid metabolism engineering; Recombinant protein; Transcription factor engineering.