Biohydrogen production through active saccharification and photo-fermentation from alfalfa

Chaoyang Lu; Yanyan Jing; Huan Zhang; Duu-Jong Lee; Nadeem Tahir; Quanguo Zhang; Wenzhe Li; Yi Wang; Xiaoyu Liang; Jian Wang; Peng Jin; Xueting Zhang

doi:10.1016/j.biortech.2020.123007

Biohydrogen production through active saccharification and photo-fermentation from alfalfa

Bioresour Technol. 2020 May:304:123007. doi: 10.1016/j.biortech.2020.123007. Epub 2020 Feb 11.

Authors

Chaoyang Lu¹, Yanyan Jing², Huan Zhang², Duu-Jong Lee³, Nadeem Tahir², Quanguo Zhang⁴, Wenzhe Li⁵, Yi Wang¹, Xiaoyu Liang², Jian Wang², Peng Jin², Xueting Zhang²

Affiliations

¹ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
² Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
³ Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
⁴ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China. Electronic address: zquanguo@163.com.
⁵ Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.

PMID: 32070841
DOI: 10.1016/j.biortech.2020.123007

Abstract

Studying biohydrogen production from alfalfa is of practical significance to cleaner production and biomass utilization. The performances of biohydrogen production through active/passive saccharification and photo-fermentation were compared. The effects of initial pH, substrate concentration, and cellulase loading on biohydrogen production from alfalfa by photosynthetic bacteria HAU-M1 were presented. It was found that the maximum hydrogen yield of 55.81 mL/g was achieved at initial pH of 6.90, substrate concentration of 31.23 g/mL, and cellulase loading of 0.13 g/g. Hydrogen yield of active saccharification and photo-fermentation was much higher as compare to passive saccharification and photo-fermentation. Initial pH value showed a more significant influence on photosynthetic bacteria in comparison to cellulase in active saccharification and photo-fermentation biohydrogen production. The low yield of propionic acid suggested that it was an efficient photosynthetic hydrogen production. Photo-fermentation hydrogen production from alfalfa provides a novel path for efficient utilization of alfalfa.

Keywords: Active saccharification; Alfalfa; Energy conversion; Hydrogen production; Photo-fermentation; Response surface methodology.

MeSH terms

Biomass
Cellulase*
Fermentation
Hydrogen
Medicago sativa*

Substances

Hydrogen
Cellulase