Modification of reduced graphene/Au-aptamer to develop an electrochemical based aptasensor for measurement of glycated albumin

Azad Farzadfard; Javad Shabani Shayeh; Mehran Habibi-Rezaei; Meysam Omidi

doi:10.1016/j.talanta.2020.120722

Modification of reduced graphene/Au-aptamer to develop an electrochemical based aptasensor for measurement of glycated albumin

Talanta. 2020 May 1:211:120722. doi: 10.1016/j.talanta.2020.120722. Epub 2020 Jan 7.

Authors

Azad Farzadfard¹, Javad Shabani Shayeh², Mehran Habibi-Rezaei³, Meysam Omidi⁴

Affiliations

¹ Protein Biotechnology Research Lab (PBRL), School of Biology, College of Science, University of Tehran, Tehran, Iran.
² Protein Research Center, Shahid Beheshti University, Tehran, Iran. Electronic address: j_shabani@sbu.ac.ir.
³ Protein Biotechnology Research Lab (PBRL), School of Biology, College of Science, University of Tehran, Tehran, Iran; Nano-Biomedicine Center of Excellence, Nanoscience and Nanotechnology Research Center, University of Tehran, Tehran, Iran. Electronic address: mhabibi@ut.ac.ir.
⁴ Protein Research Center, Shahid Beheshti University, Tehran, Iran; School of Dentistry, Marquette University, Milwaukee, USA.

PMID: 32070572
DOI: 10.1016/j.talanta.2020.120722

Abstract

Herein, an electrochemical label-free biosensor designed for the detection of glycated albumin (GA) using reduced graphene oxide/Au nanoparticles (RGO/AuNPs) modified by anti-GA aptamer. For fast and simple modification of the electrode, the aptamer chain was thiolated. Transition electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) techniques were used to the characterization of synthesized materials. Structural analysis of nanomaterials shows that graphene sheets were synthesized very fine by average thickness of 2.5 nm and Au nanoparticles distributed on the surface of graphene sheets uniformly. Cyclic voltammetry (CV) square wave voltammetry (SWV) and impedance spectroscopy (EIS) were used to electrochemical study of the decorated electrode. Electrochemical studies described the potential of fabricated rGO/AuNPs-aptamer electrode to selectively determine GA properly in buffer solution at the range of 2-10 μg mL^-1 by the detection limit of 0.07 μg. mL^-1 for GA.

Keywords: Aptamer; Electrochemical sensor; Glycated albumin; Gold nanoparticles; Reduced graphene oxide.