Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves

Chao Li; Zhuping Dong; Bin Zhang; Qiang Huang; Guang Liu; Xiong Fu

doi:10.1016/j.carbpol.2020.115897

Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves

Carbohydr Polym. 2020 Apr 15:234:115897. doi: 10.1016/j.carbpol.2020.115897. Epub 2020 Jan 27.

Authors

Chao Li¹, Zhuping Dong², Bin Zhang³, Qiang Huang³, Guang Liu⁴, Xiong Fu⁵

Affiliations

¹ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China. Electronic address: felichao@scut.edu.cn.
² School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China.
³ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China.
⁴ Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
⁵ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Joint Research Center for Tropical Health Foods, South China University of Technology, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China. Electronic address: lfxfu@scut.edu.cn.

PMID: 32070517
DOI: 10.1016/j.carbpol.2020.115897

Abstract

A novel polysaccharide, designated as MOP-3, was extracted and isolated from the leaves of Moringa oleifera. Structural characterization showed that MOP-3 had a molecular weight (MW) of 4.033 × 10⁶ Da and was composed of arabinose, glucose and galactose with a molar ratio of 47.73:1.00:57.65. Methylation and nuclear magnetic resonance (NMR) analysis revealed that the main glycosidic linkage types of MOP-3 were (1→3,6)-β-D-Galp, (1→6)-β-D-Galp, (1→5)-α-L-Araf, and T-α-L-Araf. MOP-3 containing no lipopolysaccharides showed no direct cytotoxicity to the macrophage RAW 264.7 cells within the tested concentrations. The immunomodulatory assay suggested that MOP-3 could significantly enhance pinocytic capacity and increase the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by up-regulating the corresponding mRNA expression levels in RAW 264.7 cells. Our findings demonstrated that MOP-3 could potentially be used as a functional food for the hypoimmunity therapies.

Keywords: Immunoactivity; Linkage analysis; Macrophages; Moringa oleifera; Polysaccharides.

MeSH terms

Animals
Carbohydrate Conformation
Cell Proliferation / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Mice
Moringa oleifera / chemistry*
Moringa oleifera / immunology
Plant Leaves / chemistry*
Plant Leaves / immunology
Polysaccharides / chemistry*
Polysaccharides / immunology*
Polysaccharides / pharmacology
RAW 264.7 Cells
Structure-Activity Relationship

Substances

Polysaccharides