Strain-sensitive fluorescence materials have great potential in sensing applications owing to their low cost, intuitive signal, and user friendliness. Organic crystals are one of the most developed fluorescence materials. However, modulation of the fluorescence by strain is still a challenge. Here, for the first time, we investigate the strain-sensitive fluorescence of the two-dimensional (2D) organic crystal. Without interlayer interactions, the molecular arrangement in a 2D crystal can be easily tuned, which results in photoluminescence transformation between monomer emission and excimer emission. The 2D organic crystal has higher sensitivity under strain, compared with bulk organic crystals, showing great potential in practical applications such as tactile monitors, chameleon bionic skin, and visible leakage alarms.