Profile and concentration of the low molecular weight organic acids and phenolic compounds created by two-year-old Acer platanoides seedlings growing under different As forms

Abstract

Two-year-old seedlings of Acer platanoides were cultivated during a three-month hydroponic experiment in modified Knop solution enriched with inorganic (As(III), As(V)) and organic (dimethylarsinic acid - DMA) arsenic forms at 0.06 mM, 0.6 mM and their combinations. The profile and content of low molecular weight organic acids (LMWOAs) and phenolic compounds were also determined in the rhizosphere, roots and leaves. Arsenic (As) treatment caused an elevated creation of the above mentioned metabolites, which was higher in leaves than in the rhizosphere or roots, and their overall content was correlated with the concentration of As in A. platanoides organs. The addition of all As forms strongly induced the exudation of citric and oxalic acids into the rhizosphere, while malonic, acetic, citric and malic acids were formed in the roots. The most differential profile of roots was confirmed for As(V) 0.06 mM (4-hydroxybenzoic (4-HBA), syringic, 2,5 dihydroxybenzoic (2,5-DHBA), caffeic, chlorogenic, ferulic, p-coumaric and sinapic acids and catechin). The obtained results indicate that the presence of particular As forms has a significant impact on the content and profile of exuded and created LMWOAs and phenolic compounds, and can also have a decisive influence on the activation of appropriate detoxification mechanisms.

Keywords: Acer platanoides; Arsenic accumulation; Detoxification; Organic acids; Phenolic compounds.