Objective: To analyze the association between circular RNAs expression in serum and gastric cancer and evaluate the potential of the related markers in early diagnosis of gastric cancer. Methods: Forty eight gastric cancer cases in Linqu County People's Hospital were selected as case group, and 48 controls matched by age and sex were randomly selected in the gastric cancer screening cohort during the same period. The expression levels of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 were detected by quantitative real-time PCR. The results were compared between case group and control group. Results: The positive expression rates of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 were 70.8%, 47.9%, 75.0% in case group, slightly higher than those in control group (58.3%, 31.3%, 60.4%), although P values were all more than 0.05. The expression level medians of the 3 candidate circRNAs expression levels were 1.60% (0-5.64%), 0 (0-0.61%), 0.91% (0.06%-1.88%) in case group, while 0.05% (0-6.07%), 0 (0-0.34%), 0.42% (0-1.39%) in control group, respectively. Conditional logistic regression analysis showed that the association strength of high expressions of 3 candidate circRNAs with gastric cancer showed an increase trend, but the differences had no significance after adjusted by Helicobacter pylori infection, smoking and drinking status (all P>0.05). Further analysis by combining the 3 candidate circRNAs showed the increased strength of association between circRNAs and gastric cancer with the elevated number of positive circRNAs in serum (trend test P=0.040) compared with circRNAs negative persons. Conclusion: Our study preliminarily suggested that the expression of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 in serum might be correlated with gastric cancer.
目的： 分析血清中候选环状RNA表达与胃癌的关系，初步探索其是否具有胃癌早期诊断标志物的潜在应用价值。 方法： 从山东省临朐县人民医院选取48例胃癌病例，并在同期胃镜筛查队列中选择年龄、性别匹配的48例对照。采用实时荧光定量PCR方法对血清中3个候选环状RNA（hsa_circ_002059、hsa_circ_0000096、hsa_circ_0001895）表达水平进行定量检测，并对胃癌组与对照组进行比较。 结果： 胃癌病例中血清hsa_circ_002059、hsa_circ_0000096、hsa_circ_0001895表达阳性率分别为70.8%、47.9%、75.0%，略高于对照组（58.3%、31.3%、60.4%），差异均无统计学意义（均P>0.05）。血清中上述3个候选环状RNA表达水平M（P(25)~P(75)）在胃癌组中分别为1.60%（0~5.64%）、0（0~0.61%）、0.91%（0.06%~1.88%），在对照组中分别为0.05%（0~6.07%）、0（0~0.34%）、0.42%（0~1.39%）。多因素分析调整幽门螺杆菌感染、吸烟和饮酒状况后，3种候选环状RNA高表达与胃癌之间的关联强度呈上升趋势，差异无统计学意义（均P>0.05）。进一步将上述3种环状RNA进行组合，对其与胃癌的关系进行联合分析发现，与3种环状RNA均为阴性者相比，随着表达阳性环状RNA个数增加，与胃癌的关联强度呈上升趋势，趋势检验P=0.040。 结论： 本研究初步提示hsa_circ_002059、hsa_circ_0000096、hsa_circ_0001895表达水平可能与胃癌相关。.
Keywords: Candidate biomarker; Circular RNA; Gastric neoplasm.