Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading

Hong Liu; Zhiping Zhang; Huan Zhang; Duu-Jong Lee; Quanguo Zhang; Chaoyang Lu; Chao He

doi:10.1016/j.biortech.2020.122956

Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading

Bioresour Technol. 2020 May:303:122956. doi: 10.1016/j.biortech.2020.122956. Epub 2020 Feb 4.

Authors

Hong Liu¹, Zhiping Zhang², Huan Zhang², Duu-Jong Lee³, Quanguo Zhang⁴, Chaoyang Lu¹, Chao He²

Affiliations

¹ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
² Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
³ Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
⁴ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China. Electronic address: zquanguo@163.com.

PMID: 32058909
DOI: 10.1016/j.biortech.2020.122956

Abstract

Chlorella is widely distributed, can be cultured in waste water and had short growth cycle. The high carbohydrate composition shows great potential for bioenergy output. In this work, concentrated Chlorella solution was adopted as raw material. Reducing sugar concentration, pH, and cumulative bio-hydrogen yield were taken as indexes, the effects of substrate concentration and enzyme (cellulase or neutral protease) load on photo-fermentation bio-hydrogen production process from microalgae biomass were investigated. Results showed that highest cumulative hydrogen yield was obtained at the optimal substrate concentration of 25 g/L, when the load of cellulase and protease are both 15%, the effect is the best which were 16.65 mL, 29.44 mL, and 43.62 mL, respectively. Results fitted well to the Gompertz model, indicating the feasibility of photo-fermentative bio-hydrogen production from concentrated Chlorella.

Keywords: Enzyme load; Microalgae biomass; Photo-fermentation bio-hydrogen production; Substrate concentration.

MeSH terms

Biomass
Chlorella*
Fermentation
Hydrogen
Hydrogen-Ion Concentration
Microalgae*

Substances

Hydrogen