Dihydro-β-agarofuran sesquiterpenoid derivatives with neuroprotective activity from the leaves of Tripterygium wilfordii

Peng Zhao; Bin Lin; Zi-Lin Hou; Le Zhou; Qing-Jun He; Guo-Dong Yao; Xiao-Xiao Huang; Shao-Jiang Song

doi:10.1016/j.fitote.2020.104501

Dihydro-β-agarofuran sesquiterpenoid derivatives with neuroprotective activity from the leaves of Tripterygium wilfordii

Fitoterapia. 2020 Apr:142:104501. doi: 10.1016/j.fitote.2020.104501. Epub 2020 Feb 11.

Authors

Peng Zhao¹, Bin Lin², Zi-Lin Hou¹, Le Zhou¹, Qing-Jun He¹, Guo-Dong Yao¹, Xiao-Xiao Huang³, Shao-Jiang Song⁴

Affiliations

¹ Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
² School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.
³ Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: xiaoxiao270@163.com.
⁴ Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: songsj99@163.com.

PMID: 32058050
DOI: 10.1016/j.fitote.2020.104501

Abstract

A phytochemical investigation on the leaves of Tripterygium wilfordii Hook. F. was conducted, leading to the isolation of five undescribed dihydro-β-agarofuran sesquiterpenoids (1-5) and one known analogue (6). Their structures were determined by comprehensive spectroscopic analyses. The absolute configurations of the compounds were determined by comparison of the experimental ECD with the calculated data. In addition, all the compounds were evaluated for their neuroprotective activities against H₂O₂-induced cell injury in human neuroblastoma SH-SY5Y cells, and 3 showed the better protective effect with 76.63% cell viability comparing with the positive control Trolox (69.84%) at 12.5 μM.

Keywords: Calculated ECD; Neuroprotective activity; Sesquiterpenoids; Tripterygium wilfordii.

MeSH terms

Cell Line, Tumor
Drug Evaluation, Preclinical
Humans
Neuroprotective Agents / chemistry
Neuroprotective Agents / isolation & purification*
Plant Leaves / chemistry
Sesquiterpenes / chemistry*
Tripterygium / chemistry*

Substances

Neuroprotective Agents
Sesquiterpenes
dihydroagarofuran