Loss of G-protein-signaling modulator 2 accelerates proliferation of lung adenocarcinoma via EGFR signaling pathway

Mingming Deng; Bofang Liu; Zhe Zhang; Yang Chen; Yizhe Wang; Ximing Wang; Qingjie Lv; Xianghong Yang; Kezuo Hou; Xiaofang Che; Xiujuan Qu; Yunpeng Liu; Ye Zhang; Xuejun Hu

doi:10.1016/j.biocel.2020.105716

Loss of G-protein-signaling modulator 2 accelerates proliferation of lung adenocarcinoma via EGFR signaling pathway

Int J Biochem Cell Biol. 2020 May:122:105716. doi: 10.1016/j.biocel.2020.105716. Epub 2020 Feb 11.

Authors

Mingming Deng¹, Bofang Liu², Zhe Zhang³, Yang Chen⁴, Yizhe Wang⁴, Ximing Wang⁴, Qingjie Lv³, Xianghong Yang³, Kezuo Hou⁵, Xiaofang Che⁵, Xiujuan Qu⁵, Yunpeng Liu⁵, Ye Zhang⁶, Xuejun Hu⁷

Affiliations

¹ Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, Shenyang 110001, China; Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China; Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
² Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310000, Zhejiang, China.
³ Department of Pathology, Shengjing Hospital of China Medical University, Shenyang 110001, China.
⁴ Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, Shenyang 110001, China.
⁵ Department of Medical Oncology, The First Hospital of China Medical University, Shenyang 110001, China.
⁶ The First Laboratory of Cancer Institute, The First Hospital of China Medical University, Shenyang 110001, China. Electronic address: yzhang21@cmu.edu.cn.
⁷ Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, Shenyang 110001, China. Electronic address: xjh@cmu.edu.cn.

PMID: 32058048
DOI: 10.1016/j.biocel.2020.105716

Abstract

G-protein-signaling modulator 2 (GPSM2) belongs to a protein family that regulates activation of G proteins and plays an important role in mitotic spindle orientation. However, the role of GPSM2 in lung adenocarcinoma (LUAD) is still unclear. In this study, it was found that GPSM2 correlates with clinicopathological features and patient's prognosis in LUAD. Knocking down GPSM2 promoted LUAD cell proliferation in vitro and in vivo. Mechanistically, it was demonstrated that GPSM2 knockdown accelerates cell proliferation via the EGFR pathway. These results confirmed that GPSM2 played an important role in LUAD. Moreover, GPSM2, as an independent prognostic factor, may serve as a potential drug target and prognostic biomarker in LUAD.

Keywords: Cell proliferation; EGFR; GPSM2; NSCLC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

A549 Cells
Adenocarcinoma of Lung / genetics
Adenocarcinoma of Lung / metabolism*
Adenocarcinoma of Lung / pathology
Animals
Cell Line, Tumor
Cell Proliferation / physiology
ErbB Receptors / metabolism
Heterografts
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins / deficiency*
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism
Lung Neoplasms / genetics
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Signal Transduction
Transfection

Substances

GPSM2 protein, human
Intracellular Signaling Peptides and Proteins
EGFR protein, human
ErbB Receptors