Isolation and analysis of extracellular vesicles in a Morpho butterfly wing-integrated microvortex biochip

Shanying Han; Yueshuang Xu; Jie Sun; Yufeng Liu; Yuanjin Zhao; Weiguo Tao; Renjie Chai

doi:10.1016/j.bios.2020.112073

Isolation and analysis of extracellular vesicles in a Morpho butterfly wing-integrated microvortex biochip

Biosens Bioelectron. 2020 Apr 15:154:112073. doi: 10.1016/j.bios.2020.112073. Epub 2020 Feb 7.

Authors

Shanying Han¹, Yueshuang Xu², Jie Sun³, Yufeng Liu³, Yuanjin Zhao⁴, Weiguo Tao⁵, Renjie Chai⁶

Affiliations

¹ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China.
² Department of Pathology, School of Medicine, Southeast University, Nanjing, 210009, China.
³ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
⁴ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China. Electronic address: yjzhao@seu.edu.cn.
⁵ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. Electronic address: taow@seu.edu.cn.
⁶ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; MOE Key Laboratory for Developmental Genes and Human Disease, School of Life Science and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China; Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China. Electronic address: renjiec@seu.edu.cn.

PMID: 32056968
DOI: 10.1016/j.bios.2020.112073

Abstract

With the function of mediating intercellular communication between cells, extracellular vesicles (EVs) have been intently studied for their physiopathology and clinical application values. However, efficient EV isolation from biological fluids remains a significant challenge. To address this, this work constructs a new microvortex chip that can isolate EVs efficiently by integrating the lipid nanoprobe modified Morpho Menelaus (M. Menelaus) butterfly wing into microfluidic chip. M. Menelaus wing is well known for its orderly arranged periodic nanostructures and can generate microvortex when liquid passes through it, leading to increased interaction between EVs and M. Menelaus wing. In addition, the nanoprobe containing lipid tails can be inserted into EVs through their lipid bilayer membrane structure. Based on the described properties, high-throughput enrichment of EVs with over 70% isolation efficiency was realized. Moreover, it was demonstrated that the nanoprobe system based on M. Menelaus wing enabled downstream biological analysis of nucleic acids and proteins in EVs. Microvortex chips showed potential application value in efficient EV isolation for biomedical research and cancer diagnosis.

Keywords: Biochip; Butterfly; Extracellular vesicle; Isolation; Microfluidics.

MeSH terms

Animals
Biosensing Techniques*
Butterflies / chemistry
Extracellular Vesicles / chemistry*
Extracellular Vesicles / genetics
Lab-On-A-Chip Devices
Lipid Bilayers / chemistry
Lipids / chemistry*
Nanostructures / chemistry*
Wings, Animal / chemistry

Substances

Lipid Bilayers
Lipids